The purpose of this morphological studies was to investigate the central neural pathway projecting to the acupoints B_(62) and K_6 using the neuroanatomicat method following injection of transsynaptic neurotropic virus, pseudorabies virus(PRV-Ba and PRV-Ga) into the B_(62) and K-6.
After survival times of 96 hours following injection into the twenty rats with PRV-Ba(Bartha strain) and PRV-Ga(Bartha strain, β-galacidodase insertion). They were perfused, and their spinal cord and brain were frozen sectioned(30㎛). These sections were stained by X-gal histochemical and PRV immunohistochemical staining method, and observed with light microscope.
The results were as follows : I. In spinal cord, overlaped PRV-Ba and PRV-Ga labeled neurons projecting to the B_(62) and K_6 were founded in thoracic, lumbar and sacrat spinal segments. In thoracic spinal segments, Densely labeled areas were founded in lamina IV, V, VII(intermediolateral nucleus) and X areas. In lumbar segemnts, labeled areas were founded in lamina II, IV, V and X areas. In sacral spinal segments, labeled areas were founded in lamina IV, V and VI areas.
2. In brain, overlaped PRV-Ba and PRV-Ga labeled neurons projecting to the B_(62) and K_6 were founded in the A₁, noradrenalin cells/C₁ adrenalin cells/caudoventrolateral reticular nucleus, rostroventrolateral reticular nuclens, nucleus tractus solitarius, area postrema, raphe obscurus nucleus, raphe paltidus nucleus, raphe magnus nucleus, lateral paragigantoceltular nucleus, lateral rcticular nucleus, gigantocellular nucleus, locus coeruleus, subcoeruleus nucleus, motor trigeminal nucleus, Kolliker-Fuse nucleus, A_5 cell group, central gray matter, oculomotor nerve, paraventricular hypothalamic nucleus, median eminence, amygdaloid nucleus, frontal cortex, forelimb area, hindlimb area, 1, 2 areas of parietal cortex and granular and agranular cortex.
This results were suggest that overlaped PRV-Ba and PRV-Ga labeled areas projecting to the B_(62) and K_6 may be related to the emotional relay pathway in the central autonomic center.
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